Peptide nanotubes (PNTs) are bioelectrets that exhibit piezoelectricity, pyroelectricity, ferroelectricity
and birefringence. Birefringence is an optical property that can be used to determine the degree of order,
orientation, size and shape of PNTs. In this work, a custom-built optical apparatus is used to quantitatively
measure birefringence in Three Dimensions, of PNTs under varying electric fields and temperature. This work
also includes techniques for aligning and orienting PNTs and discusses the role of birefringence measurements in
the study of PNTs.